Difference between revisions of "Arsenic Prototype 4.0"

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Revision as of 17:51, 5 January 2016

Principles

The Arsenic prototype v4.0 is the Winter School 2016 kit version of prototype 3.0.

The basis of the prototype remains the same - the bioreporter, a GMO bacteria expressing eGFP (Green Fluorescent Protein) is incubated with a water sample, where the fluorescence is detected optically and can be quantified in order to measure the concentration of Arsenic in the water

  • A vial containing the water sample we want to test is positioned on a socket through which a fluorescent excitation LED (blue 488nm for eGFP) passes.
  • GFP absorbs this blue light (λ=475 nm) and emits green light (λ=504 nm) which is detected by a photosensor on which the light is concentrated with the help of two lenses that avoid loss of intensity
  • A long-pass filter allow only the signal eGFP fluorescence to reach the photosensor


Moreover, a red LED was added to measure the transmittance, which can be converted into turbidity. The measurement of turbidity will allow us to normalize our results.

  • A red LED is placed in-line with the photosensor


We use an arduino to take the measurements, and we have a liquid crystal display (LCD) to read out the numbers.
With these measures, compared against a standard curve of water containing known arsenic concentrations, one can determine the concentration of arsenic in the sample and so know if the water is drinkable or not.



In summary, the most current version consists of:

   lasercut chassis
   blue LED for eGFP fluorescence excitation at 90 degrees from detector
   red LED for transmittance measurements in line with detector
   vial holder matching the vial approved by the Swiss authorities
   light to frequency detector
   a filter to block excitation light
   LCD screen read out
   based on the arduino



Materials


You can find the bill of materials here:

Step by Step Buildling

Laser Cutting the Inner Pieces


at chez hackuarium - laser cutter model: Keyland KQG-1060 120W CO2 laser cutter

Cutting Parameters for Material: HDF (MDF)
mode speed power scan mode interval
Groove LED (fluo) scan 50 (100) 35 x_unilat 0.1
3mm board cut 15 100
Text and Logo scan 250 (100) 25 x_unilat 0.3
Groove Lens scan 50 12 x_unilat 0.1
2mm board cut 35 100


Results



Assembling the Electronics


There are four PCBs for:

  1. Blue LED (for the eGFP excitation)
  2. LCD screen
  3. Red LED (for the transmittance)
  4. Light to frequency meter (to detect the light)

Each of the PCBs except the LCD screen PCB are fixed onto the lasercut boards using M3 screws.

Soldering and Mounting

Blue LED

Blue LED PCB and parts.png

LCD screen

LCD PCB and parts.png

Red LED

Red LED PCB and parts.png


light to frequency meter

LFD PCB and parts.png



Final Assembly

Finally, the Red LED and light to frequency meter will be plugged into the Blue LED board with connectors.
Final assembly 1.JPG

Then the LCD screen is plugged into the Blue LED board with a ribbon cable.
Final assembly 2.JPG

The LCD screen board is ready to be mounted on the arduino.

We are now ready to test it out.


Testing the Prototype



Links other References